Antinuclear Antibodies in Rabbit Antisera

Use of cultured epithelial cells, including keratinocytes, for the detection of antinuclear antibodies.

The use of cultured human epithelial cells as a substrate for the detection of antinuclear antibodies (ANAs) improves the sensitivity of detection above the standard substrates used. Standard antisera have been screened against a series of epithelial cell lines, including keratinocytes. Selected cells have then been used to detect ANAs in 78 patients with discoid lupus erythematosus.

Inhibition of Eimeria acervulina Sporozoite Invasion by Rabbit and Chicken Antisera Using ISI Assay

Detection of immunoglobulin G antibodies to cholesterol in antisera to mycoplasmas.

Antisera were produced by inoculation of mycoplasma cells grown in PPLO broth supplemented with rabbit serum alone or rabbit serum plus Freund complete or incomplete adjuvant. By using an enzyme-linked immunosorbent assay, immunoglobulin G antibodies to cholesterol were detected in antisera to mycoplasmas (Mycoplasma salivarium ATCC 23064, M. orale ATCC 15539, M. buccale IID 802, M. faucium IID...

Phosphorylase-cross-reactive antibodies evoked by streptococcal M protein.

Rabbit antisera evoked by type 5 streptococcal M protein (M5) were screened by enzyme-linked immunosorbent assay (ELISA) for immunological cross-reactivity with purified rabbit muscle phosphorylases a and b. Of 10 pep M5 antisera tested, 3 showed significant cross-reactivity with both forms of the enzyme. ELISA inhibition studies using one of the pep M5 antisera showed that all of the phosphory...

Production and Evaluation of Polyclonal Rabbit Anti-Human p53 Antibody Using Bacterially Expressed Glutathione S-transferase-p53 fusion protein

p53 is a key tumor suppressor gene that is targeted for inactivation during human tumorigenesis. In this study, we produced and characterized polyclonal antihuman p53 antibody. The cDNA encoding the completehuman p53 protein was cloned into pGEX-4T-1 and expressed in Escherichia coli as a fusion protein with Schistosoma japonicum glutathione S-transferase (GST). The rabbits were immunized...